Native SDS-PAGE: high resolution electrophoretic separation of proteins with retention of native properties including bound metal ions.

Tricine – SDS - PAGE Hermann Schägger

Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...

Two-dimensional electrophoresis of membrane proteins.

One third of all genes of various organisms encode membrane proteins, emphasizing their crucial cellular role. However, due to their high hydrophobicity, membrane proteins demonstrate low solubility and a high tendency for aggregation. Indeed, conventional two-dimensional gel electrophoresis (2-DE), a powerful electrophoretic method for the separation of complex protein samples that applies iso...

Identification of electrophoretic proteins pattern of Fusaium verticillioides isolates from maize by SDS-PAGE.

A Practical Approach on SDS PAGE for Separation of Protein

Polyacrylamide gel electrophoresis (PAGE), describes a technique widely used in biochemistry, forensics, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. Mobility is a function of the length, conformation and charge of the molecule. As with all forms of gel electrophoresis, molecu...

Analysis of Ricin Subunits by High-Resolution Acidic Native Gel Electrophoresis

Acidic gel electrophoresis of ricin subunits Abstract Ricin is a potent toxic castor bean glycoprotein comprising of two subunits, A chain and B chain. We studied ricin subunits in an acidic electrophoresis system followed by electro blotting. To the best of our knowledge, there is no study reported for characterization of ricin subunits using acidic native PAGE. Sample preparation steps for ac...